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Title:
Regulating ROS production using nanosecond pulsed plasma jets for cancer cell inactivation in vitro
Authors:
Jiang, Chunqi - Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia 23508, USA
Oshin, Edwin, A. - Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia 23508, USA
Minhas, Zobia - Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia 23508, USA
Guo, Siqi - Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia 23508, USA
Abstract:
Excessively high level of reactive oxygen species (ROS) is known to cause irreversible damage on a cellular or systemic level, whereas relatively low concentration of ROS can be beneficial to the biological system by triggering defense mechanisms that prevent cellular damage. Although this so-called “hormesis” or “mitohormesis” is based on concentrations of endogenous ROS, the concentration of exogenous ROS and the exposure time can greatly impact or directly induce the endogenous response. Nanosecond pulsed atmospheric pressure plasma jets (ns-APPJs) have been considered a promising strategy for regulated ROS production for cancer treatment. Both the gas phase and liquid phase measurements of hydroxy radicals have shown that the production of ROS can be achieved by modulating plasma treatment time, pulse voltage, duration, and repetition frequency . In addition, the properties of the biological target that the plasma is impinging on also significantly impacts the OH generation . The dosage dependence of cell viability was demonstrated with a ns-APPJ treating Pancreatic cancer (Pan02) and breast cancer (4T1-Luc) cells in vitro1. The intracellular ROS of Pan02 cells were quantitatively assessed based on the intracellular and mitochondria membrane potentials as functions of exogenous ROS in the plasma-activated media.
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* Work supported in part by the Air Force Office of Scientific Research of the United States of America (AFOSR) under award number FA9550-22-1-0428 and the Old Dominion University Seed funds.
Keywords:
nanosecond pulsed power, cold plasma, cancer treatment
Refs:
1. C. Jiang, E. B. Sozer, S. Song, N. Lai, P. T. Vernier, and S. Guo, Modulation of ROS in nanosecond-pulsed plasma-activated media for dosage-dependent cancer cell inactivation in vitro, Physics of Plasmas, 27(11), 2020, https://doi.org/10.1063/5.0020435.
2. M. Lai, S. Song, E. Oshin, L. Potter, N. Lai, and C. Jiang, “The production of OH in a nanosecond pulsed helium plasma jet impinging on water, saline, or pigskin,” Journal of Applied Physics, 131(17), May, 2022, https://doi.org/10.1063/5.0083568.
Topic 1:
13. Pulsed power devices and methods
Topic 2:
4. Diagnostics, analytics, experimental techniques
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